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Metal-coded affinity tag : ウィキペディア英語版 | Metal-coded affinity tag Metal-coded affinity tag is a method used for quantitative proteomics by mass spectrometry that uses a metal chelate complex 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetate (DOTA) coupled to different lanthanide ions. The metal complexes attach to the cysteine residues of proteins in a sample. ==Proteomic analysis== For bottom-up proteomics, the proteins can be separated by two-dimensional gel electrophoresis and analyzed by matrix-assisted laser desorption/ionization (MALDI) or electrospray ionization mass spectrometry for relative quantification or by inductively coupled plasma mass spectrometry for absolute quantification. For top-down proteomics, the undigested labeled proteins are analyzed.
抄文引用元・出典: フリー百科事典『 ウィキペディア(Wikipedia)』 ■ウィキペディアで「Metal-coded affinity tag」の詳細全文を読む
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